Exploration of specific gene expression behind the intercellular heterogeneity of type 2 cytokine secretion activity of Group 2 innate lymphoid cell (ILC2) isolated from human peripheral blood

To investigate the gene expression underlying the intercellular diversity in type 2 cytokine secretion activity of ILC2, we performed time-series measurements of type 2 cytokine secretion activity of ILC2 from human peripheral blood after IL-2/IL-33/TSLP stimulation . Single cells were collected separately before stimulation, in transition state or activated cells after stimulation, and performed RNA-seq on each cell for gene expression profiling analysis. Overall design: Comparative gene expression profiling analysis of single-cell RNA-seq data of human peripheral blood ILC2.