Time-dependent expression data from human fibrosarcoma human cell line (HT1080) in collagen matrix with protease inhibitors

HT1080 cells were grown on collagen I and treated with PI for 4 h (short term) and 24 h (long term) while RNA was extracted for Genome-wide Affymetrix Microarray gene expression analysis. The objective of this study was to identify whether collagen accumulation driven by blocking proteases induced transcriptional changes that correlated sustained amoeboid cell state. 9 samples (3 x 3 biological replicates). Cells were plated on deformable matrices. Human fibrosarcoma cells (HT1080 cells) plated on deformable matrices were left untreated or treated with proteinase kinase inhibitor cocktail.