nsp8_kurauskas

NMR spectra of SARS-CoV-2 full-length nsp8 protein: in complex with different ratios of interaction partner nsp7, as well as free nsp8 spectra at different protein and salt concentrations. Spectra include 4D methyl NOESY spectra used for the assignment of nsp8 (in nsp7-nsp8 complex). Spectra show large contrast between nsp7 bound nsp8 spectra and free nsp8 spectra. While nsp7 bound nsp8 spectra displays sharp peaks and a good correspondence with the X-ray structures, free nsp8 spectra is broad suggesting dynamic nature of nsp8. We have studied what biophysical/biological process leads to spectral broadening of nsp8, and based on this and additional data came to conclusion that nsp8 is a molten globule in its free state.