Zea mays Raw sequence reads

Purpose: The goal of this study is to identify differentially expressed genes (DEGs) in Zmer1;Zmer2, Zmer1;Zmerl and WT in maize ear primordia. Methods: the 2-5 mm ear primordia of maize Zmer1;Zmer2, Zmer1;Zmerl and WT were dissected from maize plant at the V10 stage, and samples were snap freeze using liquid nitrogen. The total RNAs were prepared using the Direct-zol RNA extraction kit (Zymo Research). All those RNA samples of Zmer1;Zmer2, Zmer1;Zmerl and WT were submitted for RNA-Seq. Conclusions: Our RNA-seq experiments identified 1305 up-regulated differential expressed genes (DEGs) and 303 down-regulated DEGs in Zmer1;Zmer2 and 1421 up-regulated DEG and 347 down-regulated DEGs in Zmer1;Zmerl (DEGs; P-value(padj) < 0.05 and log2(fold change) > 1). Overall design: RNA-seq was performed on 2-5 mm Zmer1;Zmer2, Zmer1;Zmerl and WTmaize ear primordia.