IL-1 mRNA and protein expression in freshly isolated and Ang1-stimulated neutrophils.

T0 represents content at the time of isolation and reflects the state of circulating human neutrophils in healthy individuals; intracellular and extracellular content are assessed after stimulation with Angiopoietin-1 (Ang1); S: stimulated; N.D.: not detectable. Unstimulated neutrophils do not express or store pools of IL-1α mRNA, and that is not altered by the addition of Ang1. Neutrophils hold large pools of IL-1β mRNA, but Ang1 signal is required for translation. Finally, neutrophils constitutively express and release IL-1RA.