RNA-seq analysis was performed to compare gene expression patterns between acclimated and non-acclimated plants.

We aimed to understand the ecological and evolutionary implications of thermal acclimation. Seeds of 40 maternal plants of Arabidopsis lyrata ssp. lyrata of one population were collected at Saugatuck Dunes State Park, Michigan, USA. Plants were then raised in growth chambers under three acclimation treatments: frost, heat, or no acclimation (40 maternal families x 3 treatments x 3 replicates = 360 plants). At the end of the treatment period, one rosette leaf was collected for 16 individuals per treatment for a RNA-seq analysis. We compared gene expression patterns between acclimated and non-acclimated plants.