Summary of the washing stringency conditions optimized for the different normal and mutant probes.
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Radioactive 32P labeled normal and mutant probes of Exons: 8, 10, 12, 13, &16 were hybridized with amplified denatured DNA loaded on positively charged membrane. Membranes were then washed under different stringency conditions with SSC- SDS. The abbreviation SSC-SDS stands for: Sodium chloride: Tri-Sodium Citrate (3 M: 0.3 M, pH 7) - Sodium Dodecyl Sulfate used at the indicated volume in ml, time in minutes and temperature °C.Summary of the washing stringency conditions optimized for the different normal and mutant probes.
创建时间：
2015-12-02
