H2BG53D mutation in PDAC

Study was conducted to understand the effect of the cancer associated H2BG53D mutation in PDAC. Using CRISPR/Cas9 Knock-in cell lines expressing FLAG-tagged WT and G53D mutant H2B, we conducted gene expression profilling experiments and studied the effect of the H2BG53D mutation on transcription and PDAC development. CRISPR/Cas9 cell lines expressing FLAG tagged Wildtype (WT) H2B and H2BG53D were generated to study the H2BG53D mutation. Two WT and two mutant G53D cell lines as well as the Parental S2VP10 cell lines were used for experiments. Gene expression of WT and G53D mutant lines was profiled by RNA-seq. Genomic localization of FLAG-tagged WT and G53D H2B proteins were profiled by CUT&RUN sequencing. H2A CUT&RUN experiment showed H2A occupancy. Pol 2 occupancy in the CRISPR cell lines were profiled by PRO-seq.