Cell type-specific regulation by intragenic CpG islands [RNA-seq]

We wanted to emphisize the role of intragenic CpG island in various cell differentiation. We especially examined the effect of DNA methyltransferase (DNMT) in the neural differentiation from mouse ES cell. Therefore, we performed the transcriptome analysis of mouse ES cell, ESC-derived NPC, and also in DNMT double knockout (DKO) conditions. By utilizing these result, we confirmed the genes regulated by intragenic CpG island methylation have effect in NPC differentation. Overall design: 4 RNA-seq data from mouse ES cell, mouse ES cell-derived NPC, DNMT3A/B DKO mouse ES cell, and DNMT3A/B DKO mouse ES cell-derived NPC