<i>An Apple a Day Does not Keep the Weevils Away: Enhancing Vine Weevil Monitoring with Fruit-Based Volatiles</i>

This study investigated the behavioural and electrophysiological responses of adult vine weevils to apple sauce volatiles under laboratory conditions, using Y-tube olfactometer bioassays and headspace analysis using gas chromatography coupled to mass spectrometry (GC-MS) and GC coupled to electroantennographic detection (GC-EAD). In Y-tube bioassays, more adults selected the olfactometer arm containing apple sauce volatiles compared to the control arm at lower doses (0.1 g, 1 g and 10 g) but not at higher dose (20 g). Thirteen compounds were identified in volatiles collected from apple sauce with the major components being furfural and sorbic acid. Consistent electrophysiological responses were recorded to (<i>E</i>)-2-heptenal, 1-hexanol, (<i>Z</i>)-3-hexenol and (<i>E</i>)-2-hexenol. The behavioural response of vine weevil adults to refuges baited with semiochemical lures was also tested under glasshouse conditions. In this scenario, a greater proportion of individuals were recorded in refuges baited with apple sauce compared to unbaited refuges. A similar behavioural response was also recorded when refuges were baited with a combination of apple sauce and Fortune's spindle (<i>Euonymus fortunei</i> (Turcz.) Hand.-Maz.; Celastrales: Celastraceae) compared to those that were unbaited or individually baited with Fortune’s spindle or apple sauce. This study indicates that apple sauce influences vine weevil behaviour and could serve as a basis for developing a novel lure for improved monitoring.

本研究在实验室条件下，采用Y型嗅觉仪（Y-tube olfactometer）生测法，结合气相色谱-质谱联用（GC-MS）及气相色谱-触角电位检测（GC-EAD）顶空分析技术，探究了成年葡萄象鼻虫（vine weevil）对苹果酱挥发物的行为与电生理响应。
Y型嗅觉仪生测结果显示：在低剂量（0.1 g、1 g及10 g）下，相较于对照组臂，更多成年葡萄象鼻虫选择含有苹果酱挥发物的嗅觉仪臂；但在高剂量（20 g）条件下未观测到该行为偏好。
通过顶空分析共鉴定出苹果酱挥发物中的13种化合物，其中主要成分为糠醛与山梨酸。研究记录到成虫对（E）-2-庚烯醛、1-己醇、（Z）-3-己烯醇及（E）-2-己烯醇具有一致的电生理响应。
本研究还在温室条件下，测试了成年葡萄象鼻虫对信息化合物诱饵（semiochemical lures）诱集的庇护所的行为响应。结果表明，相较于未挂诱饵的对照庇护所，挂有苹果酱诱饵的庇护所内记录到的个体比例更高。
当庇护所挂有苹果酱与扶芳藤（*Euonymus fortunei* (Turcz.) Hand.-Maz.；卫矛目：卫矛科）的组合诱饵时，相较于未挂诱饵、仅挂扶芳藤诱饵或仅挂苹果酱诱饵的庇护所，同样观测到了相似的行为偏好。
本研究表明，苹果酱可调控葡萄象鼻虫的行为，可作为开发新型监测诱饵的基础。

搜集汇总

背景与挑战

背景概述

该数据集包含葡萄象鼻虫对苹果酱挥发物的行为反应实验数据，通过Y形管嗅觉测定和温室测试，显示苹果酱在较低剂量下能有效吸引象鼻虫，并结合GC-MS分析识别了关键挥发物成分。数据集支持开发基于水果挥发物的新型监测诱饵，以增强葡萄象鼻虫的防控策略。