Study of Fe3O4 Rhodamine 6G@polydopamine Superparticles (FMSPs) on pc-12 cell line.

The primary strategy to repair the peripheral nerve injuries is to bridge the lesions by promoting axon regeneration. Thus, the ability to direct and manipulate neuronal cells axon regeneration has been one of the top priorities in the field of neuroscience. A recent innovative approach for remotely guiding neuronal regeneration is by incorporating magnetic nanoparticles (MNPs) into cells and transferring the MNPs-loaded cells into magnetic sensitive environment to response to external magnetic field. The MNPs necessary for the realization of this intent should possess several tailored features. In this study we design and prepare a type of novel Fluorescent-Magnetic bifunctional Fe3O4 Rhodamine 6G@polydopamine Superparticles (FMSPs) for neural regeneration therapeutics. By virtue of their excellent biocompatibility and ability to interact with neural cells, our homemade FMSPs can be endocytosed into cells, transported along the axons, and then aggregated in the growth cones. As a result, the mechanical forces generated by FMSPs are capable to promote the growth and elongation of axons and stimulate gene expression associated with neuron growth under the external magnetic fields. Our work demonstrates that FMSPs can be used as a novel stimulator to facilitate the non-invasive neural regeneration through cell magnetic actuation.