Investigating the impact of chronic stress on behavior, brain plasticity and hippocampal transcriptome in the ewe.

Using NGS, we report the impact of chronic stress on the transcriptome of the dorsal and ventral hippocampus in the ewe. This study identifies specific transcriptional signatures of the dorsal VS ventral hippocampus (>220 DEG) and demonstrates a very limited transcriptional impact of stress, exclusively restricted to the dorsal hippocampus (<10 DEG). Comparison of the transcriptomes of dorsal and ventral hippocampus (groups thereafer denoted "DG for dorsal" and "vHip for ventral"), in control ewes and ewes submitted to chronic stress (groups thereafter denoted "C for Control" and "St for Stress"). 6 ewes per group (C & St) / RNA individually extracted from DG and vHip of all 12 ewes / Equimolar mixes of n=2 per condition (C & St) and structure (Dg & vHip) --> Triplicates for each condition and structure.