Tcf1 preprograms glycolysis mobilization in central memory CD8+ T cells in recall response

The goal of this study is to determine the impact of Tcf1 deficiency on recall capacity of central memory CD8+ T (Tcm) cells. We demonstrate that loss of Tcf1 shows limited impact on Tcm cells at resting state, but severely comprimises activation of glycolysis and other key regulators during recall response. Based on these findings, we propose that Tcf1 preprograms the responsiveness of Tcm cells to secondary challenges. Overall design: Genome-wide expression, chromatin accessibility, TCF1 binding and chromatin interaction profiles of central memory CD8+ T cells.