S1_Fig

SI Fig. Stress sensitivity and redox state of por1△ yeast cells under oxidative stress. (A) Growth kinetics was observed in YPD medium containing 3.5 mM H2O2 by monitoring optical density at 600 nm at 2-h intervals for 32 h. Square, BY cells; circle, por1Δ cells. (B) Cell survival by streaking (upper panel) and spotting (lower panel) assays in both BY and por1Δ cells. For streaking assay, yeast cells were cultured until reaching early log-phase (A600 ≈ 1.0) and streaked onto YPD agar plates supplemented with 3.5 mM H2O2. For spotting assay, yeast cells (A600 ≈ 1.0) were exposed to 10 mM H2O2 for 1 h at 28ºC with shaking, serially diluted to 10−4 with YPD medium, and spotted onto YPD agar plates. (C) Cytosolic and mitochondrial redox states were analyzed using the indicator probes for DCFHDA and DHR 123, respectively. Yeast cells were pretreated with the probes for 20 min, washed twice with PBS, and treated with 10 mM H2O2 for 1 h with shaking. Probe intensity was visualized by fluorescence microscopy. (D) Stress sensitivity of por1Δ yeast cells to physiochemical stressors. Yeast cells (A600 ≈ 1.0) were streaked onto YPD agar plates containing various stressors as mentioned in Materials and Methods. BY, wild-type yeast cells without an empty vector; por1Δ, yeast cells in which the POR1 gene had been deleted.