SETMAR regulates gene expression and alternative splicing

Transcriptomic profiling of WT and SETMAR KO HEK293T cells. To determine how SETMAR impacts the transcriptome, CRISPR/Cas9 was used to generate HEK293T SETMAR KO cells. RNAseq was performed on two KO clones and WT cells, four replicates for each. Differential expression analysis revealed 163 genes with altered gene expression in both clones. Alternative splicing analysis revealed 255 shared alternative splicing events. Collectively, our results support a model in which SETMAR likely impacts AS through TIR-specific DNA-binding and lysine methyltransferase activities, consistent with a role in development of simian primates.