Genome-wide mapping of Notch2 target sites in the embryonic kidney.

During kidney development, Notch signaling plays multiple roles including promoting differentiation of nephron progenitors that give rise to all of the epithelial cells found in the nephron. Little is known about what genes Notch signaling regulates and how Notch signaling interacts with other transcription factors. To address this, we carried out genome-wide mapping of Notch2, a major Notch receptor required for differentiation of nephron progenitors, in the embryonic kidney. Embryonic kidneys at E16.5 were crosslinked with 1% PFA and Notch2 ChIP was performed. Sequencing libraries were generated using NEBNext ChIP-seq library prep master mix set for Illumina and sequencing was performed using the Illumina HiSeq 2000.