Effects of seed coating and priming with exogenous brassinosteroid on tobacco seed germination

Brassinosteroid (BR) is a hormone known for its crucial role in seed germination promotion. However, there is a limited understanding of potential application in tobacco seed. In this study, we investigated various active ingredients for seed coating to enhance tobacco seed germination. It revealed that seed coating with 0.0500 ppm BR and priming with 0.01 ppm BR significantly increased seed germination rates, especially in low-temperature conditions. In addition, the combined use BR with priming and seed coating exhibited a substantial improvement in seed germination rates and promoted seedling growth. Through transcriptional analysis, it found that BR treatment regulates the expression of genes associated with DNA synthesis, cell wall organization and glucan metabolic process involved in seed dormancy and seedling growth. Overall, these results provide valuable insights into the utilization of seed coating and priming techniques, particularly with the inclusion of BR, to enhance tobacco seed germination and facilitate seedling emergence.

油菜素内酯（Brassinosteroid，BR）是一类在促进种子萌发过程中发挥关键作用的植物激素。然而目前对于其在烟草种子中的应用潜力，学界尚缺乏足够的认知。本研究围绕可提升烟草种子萌发性能的多种种子包衣活性成分展开了探究。研究结果显示，使用0.0500 ppm BR进行种子包衣，以及以0.01 ppm BR进行种子引发，均可显著提升烟草种子的萌发率，该效果在低温胁迫环境下尤为显著。此外，将BR与种子引发、种子包衣技术联合使用时，可进一步显著提升种子萌发率并促进幼苗生长。通过转录分析发现，BR处理可调控与DNA合成、细胞壁构建及葡聚糖代谢过程相关的基因表达，这些过程均参与种子休眠解除与幼苗生长的调控。综上，本研究结果为优化种子包衣与引发技术的应用提供了重要参考，尤其在引入BR的情况下，可有效提升烟草种子的萌发效率并促进幼苗出苗。