Bacillus sp. BI3 Genome sequencing and assembly. Bacillus sp. BI3

The arsenic-resistant bacteria are potentially suitable for arsenic removal technologies. We report the draft genome sequence of the highly arsenic resistant Bacillus sp. BI3, with 5,637,029 bp, and with an average GC-content of 34.8%. Through the data assembly, 5,637,029 bp within 40 scaffolds were obtained, and the scaffold N50 was 612,090 bp; the longest scaffold was 1,628,050 bp.The genome of Bacillus sp. BI3 showed the mechanism of microbial As-resistance related to the presence of ars operons. The ars gene systems of Bacillus sp. BI3 involve the arsenical resistance operon repressor (ArsR), As(V) reduction to As(III) via ArsC, a cytoplasmic As(V) reductase, the arsenical pump-driving ATPase (ArsA), and the extrusion of As(III) from the cytoplasm by two membrane-associated arsenite efflux pumps: ArsB permease and Acr3-type family.Its genome sequence provides insights for setting up biotechnological application that could enhance As removal efficiency.