Blockade of IKK signaling induces RIPK1-independent apoptosis in human macrophages

Regulated cell death in response to microbial infection plays an important role in immune defense and is triggered by pathogen disruption of essential cellular pathways. Gram-negative bacterial pathogens in the Yersinia genus disrupt NF-κB signaling via translocated effectors injected by a type III secretion system, thereby preventing induction of cytokine production and antimicrobial defense. In murine models of infection, Yersinia blockade of NF-κB signaling triggers cell-extrinsic apoptosis through Receptor Interacting Serine-Threonine Protein Kinase 1 (RIPK1) and caspase-8, which is required for bacterial clearance and host survival. Unexpectedly, we find that human macrophages undergo apoptosis independently of RIPK1 in response to Yersinia or chemical blockade of IKKβ. Instead, IKK blockade led to decreased cFLIP expression, and overexpression of cFLIP contributed to protection from IKK blockade-induced apoptosis in human macrophages. We found that IKK blockade also induces RIPK1 ..., , , # Data from: Blockade of IKK signaling induces RIPK1-independent apoptosis in human cells.

We have submitted our raw data for the luminescent Caspase-Glo 3/7 Assay Kit by Promega (`Caspase-Glo.xlsx`), luminescent cell viability CellTiter-Glo 2.0 Assay Kit by Promega (`CellTiter-Glo.xlsx`), colorimetric ELISA Assay Kit by R&D Systems for the human cytokine TNFa (`ELISA_TNF_hMDM_Infection.xlsx` , `ELISA_TNF_THP-1.xlsx`) and for the human cytokine IL-18 (`ELISA_IL-18_THP-1.xlsx`), colorimetric LDH Cytotoxicity Detection Kit by Sigma (`LDH_BMDM_Infection.xlsx`, `LDH_hMDM_Infection.xlsx`, `LDH_hMDM_Stim.xlsx`, `LDH_THP-1_Stim.xlsx`), and fluorescent Propidium iodide (PI) uptake cytotoxicity assay (`PI_Uptake_hMDM`).

Apart from the PI Uptake data file (`PI_Uptake_hMDM`), each file is made up of an excel sheet with multiple tabs, starting with summarized compiled data (**Summary**), followed by data from individual experiments, labeled by date (YYYY/MM/DD). The PI Uptake data file (**PI_...