Expression data from human astrocytes (HA) under oxygen-glucose deprivation / reoxygenation. Expression data from human astrocytes (HA) under oxygen-glucose deprivation / reoxygenation

Ischemia lead to neuronal injury. Dexmedetomidine and astrocytes are likely to protect neuronal cells against ischemia-induced injury. We used microarrays to examine the gene expression variation in astrocytes activated by oxygen-glucose deprivation and reoxygenation stress and identified distinct classes of genes up- and down-regulated by dexmedetomidine pretreatment. Overall design: Human astrocytes were subjected to oxygen glucose deprivation and reoxygenation (OGD/R) stress for RNA extraction and hybridization on Affymetrix microarrays. To exam the preventive effect of DEX, cells were cultured with DEX for 1 hour, before OGD/R. Cells were washed by PBS and replaced with a glucose-free medium, set in a sealed container, filled with 95% N2 / 5% CO2, and incubated for 4 hours. The cells were then returned to air conditions, washed out and replaced with normal medium, and cells were incubated for a constant reoxygenation time. i.e., 0 h (HAC 0h and HAD 0h), 2 h (HAC 2h and HAD 2h), 6 h (HAC 6h and HAD 6h), 12 h (HAC 12h and HAD 12h), and 24 h (HAC 24h and HAD 24h)