samll RNA seq of zebrafish gonads of wildtype and pld6-/- at 22 dpf

In this study, we generated zebrafish pld6 knockouts and found that the pld6-null mutants developed as infertile males, with testes containing no germ cells. Pld6 is considered to be a candidate ribonuclease participating in primary piRNA biogenesis,which is essential for the survival of germ cells. We deep-sequenced 20-33 nt total small RNAs obtained from 22 dpf wildtype and pld6-/- gonad with three repetition. In pld6-/- gonad, small RNAs mapped to the known piRNA clusters were almost absent. Moreover, piRNA clusters in pld6-/- gonad lost the enrichment for uridine at the first position or for adenine at the 10th position. our results unequivocally demonstrate a conserved role for MitoPLD in the piRNA biogenesis pathway.