Stopped-flow analysis of SR-1 RW and GroEL R231W: dependence on prebound MDH and GroES.

A. (upper panels) Analysis of SR-1-RW initiated by addition of ATP only (left, “-GroES”) or ATP and equimolar GroES (right, “+GroES”). Black traces indicate GroEL SR-1 RW only, gray traces indicate SR-1 RW with prebound MDH. B. (lower panels) As in A., but performed using GroEL R231W instead of SR-1 RW in each experiment.

A.（上排图）仅通过添加ATP启动的SR-1-RW分析（左侧，“-GroES”）或ATP与等摩尔GroES（右侧，“+GroES”）的过程。黑色轨迹表示仅包含GroEL SR-1 RW，灰色轨迹表示带有预结合MDH的SR-1 RW。B.（下排图）与A.类似，但每个实验中使用GroEL R231W替代SR-1 RW进行操作。