Supplemental files_Paul Katongole et al.XLSX

Introduction: Uropathogenic E.coli (UPEC) remains the most common cause of urinary tract
infections (UTIs). They account for over 80-90% of all community acquired and
30-50% of all hospital acquired UTIs. E.coli
strains have been found to belong to evolutionary origins known as phylogenetic
groups. In 2013, Clermont classified E. coli strains
into eight phylogenetic groups using quadruplex PCR method. The aim of this
study was to identify the phylogenetic groups of UPEC strains in Uganda using
Clermont’s quadruplex PCR method and to assess their antibiotic susceptibility
patterns in Uganda.

Methods:
In this cross-sectional study, 140 stored
Uropathogenic E.coli isolates from the clinical microbiology laboratory,
department of Medical Microbiology, College of Health sciences Makerere
University were subjected to phylogenetic typing by a quadruplex PCR method.
Antimicrobial susceptibility testing was performed by disk diffusion method
according to CLSI 2014. Phenotypic detection of ESBL, AmpC and Carbapenemases
was done according to CLSI guidelines and Laboratory SOPs.

Results:
Phylogenetic group B2 (40%) was the most
predominant followed by A (6.23%), Clade I and II (5%), D and E (each 2.14%),
B1 (1.43%) and F and C (each 0.71%). The most common resistant antibiotic was
Trimethoprim sulphamethoxazole (90.71%) and the least was imipenem (1.43%).
73.57% of isolates were multiple drug resistant (MDR). Antibiotic resistance
was mainly detected in phylogenetic group B2 (54%).

Conclusions: Our findings showed
the high prevalence of MDR E. coli isolates with dominance of
phylogenetic group B2. About 9 % of E. coli isolates belonged to the
newly described phylogroups C, E, F, and clade I and II