Temperature effects on developing heart in mahi-mahi

Two temperature assays were made: 1) a chronic temperature exposure where embryos were raised at 26° and 30°C degrees during 48 hours and 2) an acute temperature exposure, where embryos were raised at 25°C and acutely exposed at 25, 27 and 30°C. Chronic temperature effects on cardiac function and development were assessed only after 24 and 48 hpf. Acute temperature effects were assessed all along the development during 5 days.