RNA-Seq analysis of Candida glabrata wild-type, Δckb1 and Δckb2 mutant in the presence and absence of methyl methanesulfonate (MMS).

To obtain a better understanding of molecular mechanisms underlying the DNA damage response in Δckb1 and Δckb2 mutants, transcriptome analyses were performed between WT, Δckb1, and Δckb2 strains. Comparative transcriptome analysis of Δckb1 and Δckb2 mutants showed similar transcriptional profiles in the presence and absence of MMS. The data also indicated that compared with WT, about 200 genes were up or downregulated in Δckb1 and Δckb2 strains with or without treatment with 0.01% MMS. GO analysis highlighted that the differentially expressed genes in Δckb1 and Δckb2 were significantly enriched in the processes related to the programmed formation of DNA double-strand breaks (DSBs), recombination, and DNA repair coordination of the meiotic cell cycle. The study was designed to profile the transcriptomes of C. glabrata wild-type, Δckb1 and Δckb2 mutant grown under normal (YPD medium) and DNA damage stress (0.01% MMS treatment for 1h) conditions via RNA-Sequencing approach. The experiment was performed with three biological replicates.