Transcriptomic analysis of progranulin-deficienct mouse brains

Progranulin mutations found in frontotemporal lobar degeneration (FTLD) cases typically result in heterozygous loss-of-function. To assess the impact of PGRN deficiency on gene expression, particularly of genes associated with lipid metabolism, in the brain, we sequenced the total RNA extracted from whole brains from 7-month-old female Grn+/+, Grn+/–, and Grn–/– mice and determined the differential expression of transcripts in PRGN-deficient (Grn+/- and Grn-/-) and wild type (Grn+/+) brains.