RNA-sequencing analysis gene expression changes in response to ischemia reperfusion and upon manipulation of miR-483-5p in PC12 highly differentiated cells

The consequences of cardiac arrest are often fatal, including brain injury after resuscitation. It has been reported that few people patients can recover to the neurological state before cardiac arrest. MiRNAs are short non-protein-coding RNA molecules that are evolutionarily conserved and ubiquitously expressed. Numerous pieces of research have reviewed the role of miRNAs in regulating neuronal apoptosis, regeneration, and plasticity of neurons, and inflammatory after cardiac arrest . As the stability of miRNAs in the bloodstream and the function in the regulation of neurological impairment after ischemia-reperfusion injury, microRNAs have been the most potential new biomarkers and therapeutic targets after cardiac arrest to alleviate neurological impairment . In this work, we found that the level of miR-483-5p is correlated to the prognosis of neurological function. To investigate the function of miR-483-5p on neurons after ischemia-reperfusion injury,we established highly differentiated PC12 cell lines in which miR-483-5p was overexpressed by transfection with miR-483-5p mimcis.We then performed gene expression profiling analysis using data obtained from RNA-seq of PC12 cells in different groups.心搏骤停（cardiac arrest）的结局常为致命性，复苏后还可引发脑损伤。据报道，仅有少数患者能够恢复至心搏骤停前的神经功能状态。微小RNA（miRNAs）是一类进化保守、广泛表达的非编码短RNA分子。已有大量研究综述了miRNAs在心搏骤停后调控神经元凋亡、神经元再生与可塑性以及炎症反应中的作用。鉴于miRNAs在血液中的稳定性，以及其在缺血再灌注损伤（ischemia-reperfusion injury）后神经功能缺损调控中的作用，微小RNA已成为心搏骤停后缓解神经功能缺损最具潜力的新型生物标志物与治疗靶点。本研究发现，miR-483-5p的表达水平与神经功能预后相关。为探究miR-483-5p在缺血再灌注损伤后对神经元的作用，本研究构建了高分化PC12细胞系，通过转染miR-483-5p模拟物（miR-483-5p mimics）使该分子在细胞中过表达。随后，本研究利用不同组别PC12细胞的RNA测序（RNA-seq）数据进行了基因表达谱分析。