The microbiota influences lateral root formation through ethylene response activation

To identify the mechanism of how the microbiota induces lateral root development independently of auxin signalling, we performed a transcriptional analysis using roots of wild type plants and lateral root mutants arf7 arf19, nph4-1, lbd16-1, and gnom184, in mono-association with a selection of 16 bacteria able to restore the lateral root formation in the mutants used. Comparative gene expression profiling analysis and hierarchical gene filtering of RNA-seq data for roots of wild type plants and lateral root mutans grown axenically and those exposed to each individual bacterium. We used three independent biological replicas per treatment and we repeated this experiment twice.