Proton-secreting cells modulate mucosal immune surveillance in the male reproductive tract

We identify clearly distinct transcriptomic signatures between clear cells from the caput, corpus, and cauda regions after LPS intravasal epididymal injection compared to control (saline injection). Overall design: EGFP+ clear cells were isolated by double cell sorting from the caput, corpus and cauda epididymal regions of B1 V ATPase - EGFP transgenic mice that specifically express EGFP in clear cells. Mice were subjected to in vivo intravasal epididymal injections of LPS or saline (control)