Mammalian circRNA Atlas

We developed a new pipeline for accurate circRNA detection. We build a new atlas for characterizing circRNA in human, monkey, mouse, rat and drosophila brain. Our approach allows full annotation of circRNAs with fewer false positives and negatives than any individual pipeline or combination of them. Moreover, our approach is more sensitive than any individual pipeline and allows more accurate quantification and larger number of differentially expressed circRNAs. We obtained commercial purified RNA and performed rRNA depletion. Then we treated total RNA with RNAseR and prepared a library.本研究开发了一种用于精准检测环状RNA（circRNA）的全新分析流程。我们构建了一套用于解析人、猴、小鼠、大鼠及果蝇脑组织中环状RNA的全新图谱。相较于单一分析流程或其任意组合，本方法可实现环状RNA的全面注释，且假阳性与假阴性率更低。此外，本方法的灵敏度优于所有单一分析流程，可实现更精准的定量分析，并能检测到更多差异表达的环状RNA。本研究获取了商业化纯化的RNA并进行核糖体RNA（rRNA）去除处理，随后使用RNAseR酶处理总RNA，完成了文库构建。