Global gene expression in the adult Gata6 null mouse pancreas

We report the global gene expression of mouse pancreatic cells in a pancreas-specific conditional knock-out mouse for Gata6, as compared with age-matched controls. Total RNA was extracted from the pancreas of 6-8 -week old mice of the two genotypes and analyzed. at this age, Gata6P-/- pancreata are histologically normal, but the acinar differentiation programme is already altered. we observe that loss of Gata6 causes the de-repression of ectopic non-pancreatic genes, as well as some genes involved in the mesenchymal programme. mRNA extracted from the pancreas of 4 controls and 4 Gata6P-/- mice was sequenced.本研究报道了Gata6胰腺特异性条件性敲除小鼠中胰腺细胞的全局基因表达谱，并与同年龄匹配的野生型对照小鼠进行了对比。我们从两种基因型的6至8周龄小鼠胰腺中提取总RNA并开展分析。在此年龄段，Gata6P-/-小鼠胰腺的组织学形态虽表现正常，但腺泡细胞分化程序已发生改变。研究发现，Gata6的缺失会导致异位非胰腺基因的去抑制，同时也会影响部分参与间充质程序的基因的表达。本研究对4只野生型对照小鼠与4只Gata6P-/-小鼠的胰腺提取的mRNA进行了测序。