DNA methylation analysis of Conyza canadensis.L in the native and non-native range.

To understand widespread differences in the DNA methylation patterns of Conyza canadensis leaf samples from its native and non-native ranges. Using Whole Genome Bisulfite Sequencing, we found average read coverages in high mapped reads across native and non-native samples of Conyza canadensis. Using R bioconductor package, we found enrichment score of methylated sites in both native and non-native samples. while analyzing CG, CHG and CHH methylation, we found relatively low CG and CHG methylation across transcriptional units in natives over non-natives. However, differentially methylated regions were found to be 53% hypomethylated and 41% hypermethylated in non-natives on genic regions. We selected two native samples (native rep_1, native rep_2) and two non-native samples (non-native rep_1, non-native rep_2). We then performed Whole Genome Bisulfite Sequencing on both native and non-native samples and analyzed data using R software. Here, no reference or control sample has been taken into consideration. Native samples (native rep_1, native rep_2) signify samples taken from their native habitat while non-native samples (non-native rep_1, non-native rep_2) signify samples taken from non-native habitat.