An in vitro high-content screening unveils miR-429 as a protective molecule in photoreceptor degeneration models

Transcriptome profile of optic cups from RhoP23H/+ mice following AAV-mediated subretinal delivery of pre-miR-429 or GFP (control vector) to study the molecular changes underlying the neuroprotective effect of miR-429 overexpression in an Inherited Retinal Disease (IRD) model. Total RNA was extracted at postnatal day (PN) 30 from optic cups of eyes subretinally injected at PN8 with the AAV8-miR-429 vector; RNA extracted from optic cups of controlateral eyes injected with AAV8-GFP control vector.