Figure S1 - Role of Mitochondrial Electron Transport Chain Complexes in Capsaicin Mediated Oxidative Stress Leading to Apoptosis in Pancreatic Cancer Cells

Capsaicin produces H2O2 and superoxide separately in pancreatic cancer cells. (A) and (B) BxPC-3 cells were treated with PEG-SOD (100 U/ml), PEG-catalase (500 U/ml) for 1 h followed by 150 µM capsaicin for 2 h and stained with HE and DCFDA and analysed for superoxide and hydrogen peroxide respectively. In Fig. (1C) BxPC-3 cells were treated with PEG-catalase (500 U/ml) for 1 h followed by 150 µM capsaicin for 2 h and stained HE and analysed for superoxide. *Statistically different when compared with control (P<0.05) or ** statistically different when compared with capsaicin treatment alone (P<0.05), as analyzed by one-way ANOVA followed by Bonferroni's post-hoc test. (EPS)