Expression profile of select glucose and lipid metabolic genes mediated by STIM1 knockout in SMMC7721 cells

To investigate the altered glucose and lipid metabolic genes after STIM1 knockout in SMMC7721 cells. STIM1 knockout SMMC7721 cells were constructed by CRISPR/Cas9. The cells mentioned above were cultured in DMEM supplemented with 10% (v/v) FBS at 37°C in 5% CO2. When the cells grow to 60~70% confluent, Total RNA was extracted by using Trizol (TAKARA, Japan). Reverse transcription was performed from 1μg total RNA using PrimeScript™ RT reagent Kit with gDNA Eraser (TAKARA), Quantitative real-time PCR were performed (CFX386, BioRad) with 40 cycles at 95 °C for 10 seconds, 59 °C for 20seconds and 72°C for 30 seconds. In the study presented here, Widetype (WT), STIM1 knockout (STIM1 KO)-SMMC7721 cells were used to acquire expression profiles of a total of 49 unique genes related to glucose and lipid metabolism.