Human Stress Response Specificity through MAPK Bioresonance Selectivity

In all eukaryotes, the mitogen activated protein kinase (MAPK) cascade, a multilayered interconnected network of enzymes, connects external stimuli to gene regulation, dictating cellular fate. However, mechanisms for information encoding, transmission, and decoding in this complex, fluctuating network to activate specific responses remain elusive. Here, we employ FRET to demonstrate that the central human stress regulator p38 MAPK encodes information regarding experienced stresses in oscillations of its activation state. We show that cells decode this information to drive specific responses through frequency-dependent resonance of oscillating biochemical phosphorylation reactions between p38 and downstream targets. Finally, we demonstrate how this understanding of bioresonance allows us to induce specific genetic responses simply by exposing cells to sugar to induce oscillations of p38 at predetermined frequencies. RNA-seq profiling of human HEK-293T cells that have been treated with different constant concentrations or frequency oscillations of sorbitol in order to drive p38 activation state oscillations and associated gene expression