fruitset

To determine the effects of herbivory on fruit set, we experimentally manipulated damage on 39 pairs of plants. On one plant in each pair we selected and bagged two branches with inflorescences at the bud stage that were at approximately the same size, phenological stage, and height on the plant. One of the branches received herbivore damage where one individual of Z. selenesii was placed inside the bag for three days to consume the leaves. In order to prevent the grasshopper from eating the inflorescence, the inflorescence was covered with a second bag during the three days the herbivore was feeding. The damaged branch was subsequently used to measure locally-induced responses to herbivory. The second branch of the same shrub was bagged in the same manner but received no damage and was used to measure systemically-induced responses to herbivory. The second plant in each pair served as an absolute control in which a single branch that was approximately the same size and height was bagged in the same way without placing any herbivore on the plant. After the three-day herbivore treatments, we removed the grasshoppers and the bags and allowed natural pollinator visitation to the inflorescences. We counted the total number of flowers that were aborted and the number that successfully set fruit. The first column (replica) show the number of the replica. The second column shows the treatment of the branch. The third column shows the number of fruit that was developed in the branch. the fourth column shows the number of flowers in the branch. the fifth column shows the fruit set (proportion of flowers that becomes in fruits).

为明确植食作用对坐果率的影响，我们针对39组植株开展了损伤操控实验。每组包含两株植株，每株选取两个处于花苞期、大小、物候阶段及植株着生高度大致一致的带花序枝条并套袋。其中一个枝条施加植食性损伤：将一头Z. selenesii个体置于袋内三天以取食叶片。为防止蝗虫取食花序，在蝗虫取食的三天期间，需使用第二层袋覆盖花序。该受损枝条后续用于测定植食作用引发的局部诱导响应。同一植株的另一枝条以相同方式套袋但未施加损伤，用于测定植食作用引发的系统诱导响应。每组的第二株植株作为绝对对照组，选取一个大小、着生高度大致相当的枝条以相同方式套袋，不放置任何植食性昆虫。三天植食处理结束后，移除蝗虫与套袋装置，允许传粉昆虫自然访问花序。统计败育的总花数及成功坐果的数量。数据集第一列（replica）为重复组编号；第二列为枝条所接受的处理；第三列为该枝条发育出的果实数量；第四列为该枝条的总花数；第五列为坐果率（即发育为果实的花朵占比）。