Lable- Free protein mass spectrometry for ht-SKPs-EV and FBs-EV

We apply 3D directed induced transfer culture system alongsideand ultra-high speed centrifugation technology to break through the mass production bottleneck of ht-SKPs-EV (human transformed skin-derived precursors). We hope to compare its protein expression with that of fibroblasts extracellular vesicles (FBs-EV) in traditional adherent culture mode.The experimental procedure for analyzing ht-SKPs-EV and FBs-EV proteins using proteomics involves extracting proteins from ht-SKPs-EV and FBs-EV, subjecting them to gel electrophoresis, transferring the proteins onto a membrane, probing with specific antibodies, and detecting the protein bands to assess expression levels.