Pushing the Boundaries of Resolution in Solid-State Nuclear Magnetic Resonance of Biomolecules with 160 kHz Magic-Angle Spinning

We present ultrafast magic-angle spinning (MAS) at 160 kHz in solid-state nuclear magnetic resonance (NMR), demonstrating unprecedented spectral quality and coherence lifetimes in proton-detected experiments on biomolecular systems. By optimizing experimental and sample conditions and utilizing advanced filling and handling tools, we achieve superior resolution on both microcrystalline and membrane-reconstituted proteins, paving the way for new applications in structural biology.