Biochemical analysis of the cell surface expression of hDAT and hNET variants in the transfected COS-7 cells.

COS-7 cells transfected with FL hDAT and hDATΔEX6 (A), or FL hNET and hNETΔEX6 (B) were incubated with sulfo-NHS-SS-biotin for 60 min at 4°C, and then processed for the isolation of biotinylated DAT or NET proteins in the plasma membrane (PM) or total fraction (T). Western blotting with hDAT- or hNET-specific antibody demonstrated the cell surface expression of 80–75 kDa and 50–45 kDa hDATΔEX6 or 80 kDa and 55–50 kDa hNETΔEX6 in association with FL hDAT or FL hNET, respectively. Data represent a typical result of at least 3 repetitive experiments.