Enhanced IFN-γ secretion by PBMC co-cultivated with LB1319-MEL cells pre-treated in vitro with hIFN-γ+GGTI-298.

PBMC from 3 HLA-A0201 HD were stimulated twice in vitro with LB1319-MEL cells untreated (NT); or pretreated with 50 UI/mL hIFN-γ (IFN); 10 µM GGTI-298 (GGTI); or the combination of 50 UI/mL hIFN-γ and 10 µM GGTI-298 (IFN+GGTI) as indicated. 24h after the second in vitro stimulation, cytokine secretions in the culture supernatants were measured by Multi-Plex human cytokine assays. Results are illustrated in pg/mL for secretions of IFN-γ, IL-2, IL-4 and IL-10. Data are shown as mean (+/− standard deviation) of duplicate values and were obtained from one donor representative of the two others giving comparable results.