Data_Sheet_1_A Genome-Wide Association Study Identifies New Loci Involved in Wound-Induced Lateral Root Formation in Arabidopsis thaliana.PDF

Root systems can display variable architectures that contribute to nutrient foraging or to increase the tolerance of abiotic stress conditions. Root tip excision promotes the developmental progression of previously specified lateral root (LR) founder cells, which allows to easily measuring the branching capacity of a given root as regards its genotype and/or growth conditions. Here, we describe the natural variation among 120 Arabidopsis thaliana accessions in root system architecture (RSA) after root tip excision. Wound-induced changes in RSA were associated with 19 genomic loci using genome-wide association mapping. Three candidate loci associated with wound-induced LR formation were investigated. Sequence variation in the hypothetical protein encoded by the At4g01090 gene affected wound-induced LR development and its loss-of-function mutants displayed a reduced number of LRs after root tip excision. Changes in a histidine phosphotransfer protein putatively involved in cytokinin signaling were significantly associated with LR number variation after root tip excision. Our results provide a better understanding of some of the genetic components involved in LR capacity variation among accessions.

根系结构可展现出多样化的架构，这些架构有助于营养物质的觅食或增强对非生物胁迫条件的耐受性。通过根尖切除，可促进先前已指定的侧根（LR）原基细胞的发育进程，从而便于测量特定根系在基因型和/或生长条件下的分支能力。在本研究中，我们描述了在根尖切除后，120个拟南芥（Arabidopsis thaliana）基因型在根系结构（RSA）方面的自然变异。利用全基因组关联分析，发现与RSA变化相关的19个基因组位点。对与伤口诱导的LR形成相关的三个候选位点进行了研究。由At4g01090基因编码的假想蛋白的序列变异影响了伤口诱导的LR发育，其功能缺失突变体在根尖切除后表现出LR数量减少。参与细胞分裂素信号传导的组氨酸磷酸转移蛋白的变化与根尖切除后LR数量的变异显著相关。我们的研究结果加深了对基因型之间LR能力变异所涉及的遗传成分的理解。