<i>Francisella tularensis</i> live vaccine strain immunoreactive proteins.

(a)Locus tag from SCHU S4 database. This corresponds to numerically annotated immunoreactive areas in Figure 1.
(b)Indicates whether protein reactivity was observed in DVC LVS total soluble proteome extract (TP) or membrane enriched proteome (M).
(c)Indicates whether immunoreactivity was observed towards each protein was observed with immune sera from LVS vaccinated BALB/c or C57BL/6 mouse strains. From–(no reactivity), (+/−) at the limits of detection, to ++++ (intense reactivity).
(d)PSORT–predicted subcellular location. COG- Clusters of Orthologous groups, functional annotation based upon protein sequence. OM indicates ‘outer membrane’.
(e)Number indicates reference in which protein immunoreactivity was previously reported. ‘M’ or ‘H’ indicate whether the reported study used sera drawn from murine models of tularemia (M) or human subjects (H).
*Total intensity for all immunoreactive areas identified as FopA. Details of scoring for protein identification by using tandem mass spectrometry are shown in supplementary table S1.