Efficacy of Lactiplantibacillus plantarum PBS067, Bifidobacterium animalis subsp. lactis BL050, and Lacticaseibacillus rhamnosus LRH020 in the amelioration of vaginal microbiota in post-menopausal women: a prospective observational clinical trial

Background: The menopausal transition marks a significant physiological shift in women, followed by hormonal fluctuations and various physical and psychological changes. Menopause-related symptoms, can significantly affect a woman's quality of life and probiotics, known for their potential, have emerged as a promising avenue. This study aims to investigate the potential benefits of probiotics in ameliorating and improving vaginal well-being and vaginal microbiota composition in menopausal women.Methods: A prospective observational clinical trial (ISRCTN15737648) was carried-out enrolling 50 post-menopausal healthy women, aged between 45 and 65 years old. Women were asked to take a supplement containing Lactiplantibacillus plantarum PBS067, Bifidobacterium animalis subsp. lactis BL050 and Lacticaseibacillus rhamnosus LRH020 (3B CFU/day) for 28 days. Vaginal swabs to evaluate vaginal microbiota fluctuation as well as the vaginal inflammatory pattern have been collected. Vaginal Health Index (VHI) have been provided to evaluate vaginal well-being throughout the trial.