Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and Osmr-/- Epidermal keratinocytes Transcriptomes

Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to compare epidermal keratinocytes transcriptome profiling (RNA-seq) between wild type and Osmr-/- mice Methods: Epidermal keratinocytes mRNA profiles of wild-type (WT) and Oncostatin M receptor (Osmr-/-) mice were generated by deep sequencing, in triplicate. The sequence reads that passed quality filters were analyzed at the transcript isoform level with two methods: Burrows-Wheeler Aligner (BWA) followed by ANOVA (ANOVA) and TopHat followed by Cufflinks. Epidermal keratinocytes mRNA profiles of wild type (WT) and Osmr-/- mice研究背景与目的：下一代测序（Next-generation sequencing, NGS）已彻底革新了基于系统生物学的细胞通路分析范式。本研究旨在对比野生型与抑瘤素M受体基因敲除（Osmr-/-）小鼠的表皮角质形成细胞转录组谱（RNA-seq）。实验方法：本研究通过深度测序构建野生型（WT）及抑瘤素M受体（Oncostatin M receptor, Osmr-/-）小鼠的表皮角质形成细胞mRNA表达谱，每组设置三次生物学重复。对通过质量过滤的测序读段，分别采用两种分析流程在转录本异构体水平开展解析：其一为Burrows-Wheeler比对工具（Burrows-Wheeler Aligner, BWA）结合方差分析（ANOVA），其二为TopHat与Cufflinks联用分析。