Elevated atmospheric CO2 concentration decreases prokaryotic diversity in the rhizosphere of barley (Hordeum vulgare L.)

Barley (Hordeum vulgare L.) was grown under ambient (400 ppm) or elevated (700 ppm) atmospheric CO2 concentration in a field experiment using open-top chambers. The root associated microbiota of the plants were sampled and the samples subjected to PCR targeting the V4 region of the 16S rRNA gene using primers S-D-Arch-0519-a-S-15 and S-D-Bact-0785-a-A-21. PCR products were sequenced on an Illumina MiSeq instrument with 500 cycle v2 kit to obtain 251 bp long paired-end reads.