An injury responsive enhancer promotes heart regeneration in zebrafish

we profiled chromatin accessibility of zebrafish endothelial cells after amputation injury and identify an mmp14b endothelial enhancer induced by heart injury in zebrafish. We show that the enhancer is required for mmp14b expression and regeneration in response to heart injury. Moreover, the enhancer depends on a consensus TEAD-binding motif, suggesting a role for Hippo signaling upstream of mmp14b. Overall design: After cardiac injury, Fli1+ cells are purified for ATAC-seq profiling.