Suuporting data for Polyphenol Schisandrin B as an alternative treatment for colorectal cancer

Suuporting data for Polyphenol Schisandrin B as an alternative treatment for colorectal cancer. To evaluate the anti-cancer effects of Sch B in human colorectal cancer (CRC), an array of CRC cell lines were treated with Sch B. Selected cell lines were evaluated for their cell viability, proliferation, cell cycle status, apoptosis status, and migratory potential in response to Sch B treatment. Sch B was found to lower CRC cell viability significantly, by inhibiting cell proliferation, inducing cell cycle arrest and apoptosis. Also, the anti-cancer molecular mechanism of Sch B was elucidated using RNAseq analysis, followed by qPCR and western blot. These assays suggested Sch B achieved its anti-cancer effects by upregulating CHOP pathway and inducing ER stress in the cancer cells.The anti-cancer effects of Sch B were also validated in animal models, using zebrafish embryos and nude mice. Sch B significantly reduced transplanted tumour size by around 47% in the mouse model. However, the anti-cancer mechanisms in the in mouse model was different from the cell culture model. Instead of upregulating CHOP signaling, Sch B promoted LXR signaling, which in turn led to apoptosis of the tumour cells.