RNASeq to investigate ETV4 and ETV5 function in human foetal lung tip progenitor cells

In order to understand transcription factor ETV4 and ETV5 function, we have knocked down ETV4 and ETV5 synergistically in human foetal lung tip progenitor organoids using an inducible CRISPRi system. We discovered that ETV double knockdown led to organoid cell self-renewal defects We used 2 independent organoid parental lines infected with an inducible CRISPRi system. 2 different non-targeting control (NT) gRNAs, which do not bind to anywhere of the genome, 2 gRNA combinations targeting for ETV4 and ETV5 double knockdown, were used to control non-specific effect from organoid lines, lentiviral infection and different gRNAs.