MEK inhibitor – single-cell feature values

Data in the paper "Cellular state landscape and herpes simplex virus type 1 infection progression are connected" by Pietilä et al., 2023:
- Single-cell mean intensity of p-ERK: HeLa_MEKInhibitor_pERK.csv (Supplementary Figure 10a)
- Summary of ICP27-expressing HeLa cells: HeLa_MEKInhibitor_ICP27_summary_twoReplicates.csv (Figure 5d)

HeLa cells were infected with HSV-1 and treated with a MEK inhibitor (UO126) or DMSO (indicated by “treatment”), and after fixation at 12 or 18 hpi (indicated by “timePoint”) p-ERK and ICP27 were detected by immunofluorescence imaging. Cells were classified into uninfected cells without infected neighbors, uninfected cells with infected neighbors, and infected cells (indicated by “classification_infection”) as described in the paper. Single-cell intensities are provided as background-subtracted mean intensity values for the whole-cell. Data cleanup and background subtraction of the intensity values were applied as described in the paper. Experimental conditions are in duplicate or triplicate (indicated by “well_name”). Summary of ICP27-expressing HeLa cells reports not-normalised values.